Abstract：Objective: To investigate the effects of MACC1 on proliferation and invasion of T24 cells. Methods: MACC1 gene coding region was cloned into lentivirus vector, and lentivirus particles were infected into the human bladder carcinoma cell line T24 to upregulate the expression of MACC1 gene. The up-regulated efficiency of targeting MACC1 gene at mRNA level was detected by real-time quantitative PCR, the effect on proliferation of T24 cells was assayed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and the invasion ability was detected by transwell motility assay. Results: The lentiviral vector targeting MACC1 gene was constructed successfully, and a stable human bladder cancer cell line T24 line that up-regulated MACC1 was established. Quantitative real-time PCR and Western blotting results showed that the expression of MACC1, HGF, and c-Met gene was efficiently up-regulated by infecting LV-MACC1-GFP (P<0.05). The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and growth curve showed that the over-expression of the MACC1 gene successfully increased the proliferative capability of T24 cells. The transwell assay also showed similar increasing results on the migration ability (P<0.001). The number of transmembrane cells in LV-MACC1-GFP group was 230.3% of blank control group. Conclusions: The MACC1 gene plays a significant role in the proliferation and migration abilities of human bladder cancer cell line T24.
[1]Wang G, Fu Z, Li D. MACC1 overexpression and survival in solid tumors: a meta-analysis. Tumour Biol, 2015,36(2):1055-1065. [2]龙俊任,董自强,熊飞,等.膀胱癌组织中MACC1、c-Met表达变化及意义.山东医药,2012,52(18):74-75. [3]Stein U, Walther W, Arlt F, et al. MACC1, a newly identified key regulator of HGF-MET signaling, predicts colon cancer metastasis. Nat Med, 2009,15(1):59-67. [4]Ge Y, Meng X, Zhou Y, et al. Positive MACC1 expression correlates with invasive behaviors and postoperative liver metastasis in colon cancer. Int J Clin Exp Med, 2015,8(1):1094-1100. [5]Birchmeier C, Birchmeier W, Gherardi E, et al. Met, metastasis, motility and more. Nat Rev Mol Cell Biol, 2003,4(12):915-925. [6]Sueta A, Yamamoto Y, Yamamoto-Ibusuki M, et al. Differential role of MACC1 expression and its regulation of the HGF/cMet pathway between breast and colorectal cancer. Int J Oncol, 2015,46 (5):2143-2153. [7]Arlt F, Stein U. Colon cancer metastasis: MACC1 and Met as metastatic pacemakers. Int J Biochem Cell Biol, 2009,41(12):2356-2359. [8]Sheng XJ, Li Z, Sun M, et al. MACC1 induces metastasis in ovarian carcinoma by upregulating hepatocyte growth factor receptor c-MET. Oncol lett, 2014,8(2):891-897. [9]魏晓龙,郭和清.晚期膀胱癌化疗进展.微创泌尿外科杂志,2013,2(2):146-150. [10]You H, Ding W, Dang H, et al. c-Met represents a potential therapeutic target for personalized treatment in hepatocellular carcinoma. Hepatology, 2011,54(3):879-889.
